K array of cells

Metrics details. Microarray analyses identified a total of genes that were differentially expressed in e The expression of one of these, Spondin 1 , which encodes an extracellular matrix protein, has not previously been described in the pancreas. Several of the differentially expressed genes were known to be important for pancreatic progenitor cell proliferation and differentiation whereas others have not previously been associated with pancreatic development. The pancreas is an endodermally derived organ that forms from a ventral and a dorsal evagination of the foregut epithelium. These two evaginations, the dorsal and ventral pancreatic buds, subsequently grow, branch and differentiate into distinct pancreatic cell types [ 1 ].

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K array of cells

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Content:

• Array Formula Examples – Simple to Advanced
• Cell Adhesion Assays, ECM Array
• Detection of circulating cancer cells with K-ras oncogene using membrane array
• Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction
• Cellular automaton
• CREATE AN ARRAY
• Place k elements such that minimum distance is maximized
• K-Array Pinnacle KR802 I Portable Line Array System
• Array Functions

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Array Formula Examples – Simple to Advanced

These metrics are regularly updated to reflect usage leading up to the last few days. Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts. The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.

Find more information on the Altmetric Attention Score and how the score is calculated. Cell aggregates have attracted much attention owing to their potential applications in tissue engineering and drug screening. To evaluate cellular respiration of individual cell aggregates in these applications, noninvasive and on-chip high-throughput analytical tools are necessary. Electrochemical methods for detecting oxygen concentrations are useful because of their noninvasiveness.

However, these conventional methods may be unsuitable for high-throughput detection because it is difficult to prepare many electrodes on a small chip owing to the limitation of area for connecting electrodes.

Alternatively, a bipolar electrode BPE system offers clear advantages. In this system, electrochemical reactions are induced at both ends of a BPE without complex wiring. In this study, we present a BPE array for detecting the respiratory activity of cell aggregates.

To separate ECL chemicals from cell aggregates, we fabricated a closed BPE device containing analytical and reporter chambers. As a proof of concept, 32 BPEs were controlled wirelessly using a pair of driving electrodes, and the respiratory activities of individual MCF-7 cell aggregates as a cancer model were successfully detected by monitoring ECL signals. Compared with conventional electrode arrays for cell analysis, the wiring of the current device was simple because the multiple BPEs functioned with only a single power supply.

To the best of our knowledge, this is the first study of on-chip analysis of cellular activity using a BPE system. Additional discussion of redox currents during ECL measurements, overview of the detection system, the fabrication process for the closed BPE array, the raw image of Figure 4 A, current vs E tot , and the phase-contrast image of the cell aggregate PDF. Such files may be downloaded by article for research use if there is a public use license linked to the relevant article, that license may permit other uses.

More by Kosuke Ino. More by Ryosuke Yaegaki. More by Kaoru Hiramoto. More by Yuji Nashimoto. More by Hitoshi Shiku. Cite this: ACS Sens. Article Views Altmetric -. Citations Supporting Information. Cited By. This article is cited by 19 publications. Kira L. Rahn, Robbyn K. Analytical Chemistry , 93 1 , Anderson, Peter A. Defnet, Bo Zhang. Analytical Chemistry , 92 9 , A multicolor electrochemiluminescence device based on closed bipolar electrode for rapid visual screening of Salmonella typhimurium.

Sensors and Actuators B: Chemical , , Advanced Materials Technologies , 27 , Bipolar electrochemiluminescence sensors: From signal amplification strategies to sensing formats.

Coordination Chemistry Reviews , , Measurement methods of single cell drug response. Talanta , 15 , In vitro electrochemical assays for vascular cells and organs. Tetrahedron , 99 , Inoue , Hitoshi Shiku. ChemElectroChem , 8 18 , Light in Electrochemistry. Electrochem , 2 3 , Watts , Aleksandr Noy , Frankie J.

Small , 17 32 , Inoue , Yuji Nashimoto , Hitoshi Shiku. Bipolar Bio electroanalysis. Annual Review of Analytical Chemistry , 14 1 , Development of a novel sensing platform based on molecularly imprinted polymer and closed bipolar electrochemiluminescence for sensitive detection of dopamine. Journal of Electroanalytical Chemistry , , Tyramide signal amplification and enzyme biocatalytic precipitation on closed bipolar electrode: Toward highly sensitive electrochemiluminescence immunoassay.

Bipolar electrochemistry in synergy with electrophoresis: electric field-driven electrosynthesis of anisotropic polymeric materials. Chemical Communications , 56 92 , Bioimaging using bipolar electrochemical microscopy with improved spatial resolution. The Analyst , 21 , Oxygen consumption rate of tumour spheroids during necrotic-like core formation.

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Cell Adhesion Assays, ECM Array

These metrics are regularly updated to reflect usage leading up to the last few days. Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts. The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online.

Detection of circulating cancer cells with K-ras oncogene using membrane array

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Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction

Advances in Oscillating Reactions View all 11 Articles. We examine dynamical switching among discrete Turing patterns that enable chemical computing performed by mass-coupled reaction cells arranged as arrays with various topological configurations: three coupled cells in a cyclic array, four coupled cells in a linear array, four coupled cells in a cyclic array, and four coupled cells in a branched array. Each cell is operating as a continuous stirred tank reactor, within which the glycolytic reaction takes place, represented by a skeleton inhibitor-activator model where ADP plays the role of activator and ATP is the inhibitor. The mass coupling between cells is assumed to be operating in three possible transport regimes: i equal transport coefficients of the inhibitor and activator ii slightly faster transport of the activator, and iii strongly faster transport of the inhibitor. Each cellular array is characterized by two pairs of tunable parameters, the rate coefficients of the autocatalytic and inhibitory steps, and the transport coefficients of the coupling.

Cellular automaton

A cellular automaton pl. CA is a discrete model of computation studied in automata theory. Cellular automata are also called cellular spaces , tessellation automata , homogeneous structures , cellular structures , tessellation structures , and iterative arrays. A cellular automaton consists of a regular grid of cells , each in one of a finite number of states , such as on and off in contrast to a coupled map lattice. The grid can be in any finite number of dimensions. For each cell, a set of cells called its neighborhood is defined relative to the specified cell.

CREATE AN ARRAY

Aaron M. Elliott, Kristi A. Culturing stem cells for an extended period of time can lead to acquired chromosomal aberrations. Determining the copy number variant CNV profile of stem cell lines is critical since CNVs can have dramatic effects on gene expression and tumorigenic potential. Here, we describe an improved version of our StemArray, a stem-cell-focused comparative genomic hybridization aCGH microarray, which contains , probes and covers over stem cell and cancer related genes at the exon level. We have dramatically increased the median probe spacing throughout the genome in order to obtain a higher resolution genetic profile of the cell lines. To illustrate the importance of using the StemArray, we describe a karyotypically normal iPSC line in which we detected acquired chromosomal variations that could affect the cellular phenotype of the cells.

Place k elements such that minimum distance is maximized

As availability of primary cells can be limited for genetic studies of human disease, lymphoblastoid cell lines LCL are common sources of genomic DNA. To test for genotypic errors potentially induced by the Epstein-Barr Virus transformation process, we compared single nucleotide polymorphism SNP genotype calls in peripheral blood mononuclear cells PBMC and LCL from the same individuals. The average mismatch rate across 19 comparisons was 0. Mismatch rates were not correlated across genotype subarrays run on all sample pairs.

K-Array Pinnacle KR802 I Portable Line Array System

Documentation Help Center Documentation. A cell array is a data type with indexed data containers called cells , where each cell can contain any type of data. Cell arrays commonly contain either lists of text, combinations of text and numbers, or numeric arrays of different sizes. Refer to sets of cells by enclosing indices in smooth parentheses,. To create a cell array with a specified size, use the cell function, described below.

Array Functions

Nikolas H. The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors. Only few selected cancer cells drive tumor progression and are responsible for therapy resistance. Their specific genomic characteristics, however, are largely unknown because high-resolution genome analysis is currently limited to DNA pooled from many cells. Here, we describe a protocol for array comparative genomic hybridization array CGH , which enables the detection of DNA copy number changes in single cells. Combining a PCR-based whole genome amplification method with arrays of highly purified BAC clones we could accurately determine known chromosomal changes such as trisomy 21 in single leukocytes as well as complex genomic imbalances of single cell line cells.

Submit a Pull Request Report a Bug. Compares both value and index. Ignores value of array, compares only indexes. Compares values only.